Jove
Visualize
Contact Us

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.0K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.4K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.4K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.6K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.6K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.1K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.1K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

26.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
26.1K
Lineage Commitment01:21

Lineage Commitment

3.8K
Commitment is the  process whereby stem cells:
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A syngeneic spontaneous zebrafish model of <i>tp53</i>-deficient, EGFR<sup>vIII</sup>, and PI3KCA<sup>H1047R</sup>-driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapse in vivo.

eLife·2024
Same author

The BAF chromatin remodeling complex licenses planarian stem cells access to ectodermal and mesodermal cell fates.

BMC biology·2023
Same author

aristaless-like homeobox-3 is wound induced and promotes a low-Wnt environment required for planarian head regeneration.

Development (Cambridge, England)·2023
Same author

Finding the potency in planarians.

Communications biology·2022
Same author

Collagen IV differentially regulates planarian stem cell potency and lineage progression.

Proceedings of the National Academy of Sciences of the United States of America·2021
Same author

The DEAD-box helicase DDX56 is a conserved stemness regulator in normal and cancer stem cells.

Cell reports·2021
Same journal

Varying susceptibility of subpopulations along the epithelial-mesenchymal spectrum to undergo EMT.

The FEBS journal·2026
Same journal

ALOX15 links lipid metabolism to receptor trafficking in platelet activation.

The FEBS journal·2026
Same journal

A two-component ortholog of the Niemann-Pick C 1 protein is essential for normal growth and sterol trafficking and signaling in Tetrahymena thermophila.

The FEBS journal·2026
Same journal

MMP9 drives cancer invasion by mediating integrin membrane trafficking and stabilization.

The FEBS journal·2026
Same journal

The 2026 Richard Perham Prize winner: Angela M. Gronenborn.

The FEBS journal·2026
Same journal

Circadian rhythms of metabolite abundance in Drosophila are largely driven by time of feeding.

The FEBS journal·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 5, 2025

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Published on: April 7, 2008

36.7K

One stem cell program to rule them all?

Mallory Wiggans1,2, Bret J Pearson1,2,3

  • 1Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, Canada.

The FEBS Journal
|October 16, 2020
PubMed
Summary
This summary is machine-generated.

Stem cells, exhibiting self-renewal and multipotency, are ancient animal features. Comparing stemness gene signatures across species can reveal ancestral programs, with their dysregulation linked to cancer stem cells.

Keywords:
cancer stem cellsevolutionplanariansstem cells

More Related Videos

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.5K
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

6.9K

Related Experiment Videos

Last Updated: Dec 5, 2025

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
13:02

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Published on: April 7, 2008

36.7K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.5K
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

6.9K

Area of Science:

  • Evolutionary Biology
  • Developmental Biology
  • Genetics

Background:

  • Stem cells, characterized by self-renewal and multipotency, are present in many animal species, suggesting an ancient evolutionary origin.
  • Identifying and comparing stemness gene signatures across diverse animals and evolutionary distances presents significant challenges.
  • The concept of 'stemness' may involve conserved, ancestral genetic circuitry across the animal kingdom.

Purpose of the Study:

  • To explore the hypothesis that stemness, defined by self-renewal and multipotency, is governed by ancestral genetic programs.
  • To outline a framework for identifying and comparing stemness gene signatures across diverse animal species.
  • To investigate the evolutionary basis of stem cell function and its potential link to cancer.

Main Methods:

  • Experimental validation of self-renewal and multipotency in different cell types.
  • Genomic analyses to identify and compare gene expression profiles in stem cells versus non-stem cells.
  • Bioinformatic approaches to map gene homology and identify conserved genes across diverse animal taxa.
  • Functional assays to test the role of conserved genes in stem cell maintenance and function.

Main Results:

  • The study proposes a multi-step methodology to identify conserved stemness genes across evolution.
  • It highlights the necessity of experimental validation and comparative genomics for uncovering ancestral stemness programs.
  • The research suggests a potential evolutionary link between stemness gene dysregulation and the development of cancer stem cells.

Conclusions:

  • Comparing stem cell-specific gene signatures across evolutionary history can reveal fundamental, ancestral programs of stemness.
  • Understanding these conserved genetic circuits is crucial for deciphering the origins of stem cell biology.
  • Dysregulation of these ancestral stemness programs is implicated in the biology of cancer stem cells.