Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pinching-off of Coated Vesicles

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Family carer's experience of a delirium resource (PREDICT) to support care partnerships with healthcare professionals: A qualitative study.

Geriatric nursing (New York, N.Y.)·2026

Same author

The Michigan Sibling Immunity Birth Study (M-SIBS): Study design protocol for a unique food allergy birth cohort.

The journal of allergy and clinical immunology. Global·2026

Same author

Stoichiometrically Defined Antibody-DNA Conjugates for Quantitative Super-Resolution Imaging.

Nano letters·2026

Same author

A Scoping Review of Technology/Digital Support Tools for Informal Mental Health Carers: Impacts on Well being and Experience.

Community mental health journal·2026

Same author

Quantifying interleaflet coupling of phase behavior and observing anti-registered phases in asymmetric lipid bilayers.

Biophysical journal·2026

Same author

Comparison of baseline TEWL values in OFC positive and negative patients.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026

Search research articles

Related Experiment Video

Updated: Dec 5, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

FRET from phase-separated vesicles: An analytical solution for a spherical geometry.

Haden L Scott¹, James R Baker², Aaron J Frederick²

¹Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37920, United States; Shull Wollan Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States; Department of Chemistry, University of Tennessee, Knoxville, TN 37996, United States.

Chemistry and Physics of Lipids

|October 16, 2020

Summary

Förster resonance energy transfer (FRET) models predict size-dependent efficiency in phase-separated lipid bilayers due to domain size. However, experiments showed no significant FRET efficiency change with vesicle size, suggesting masking effects in extruded samples.

Keywords:

Förster resonance energy transfer Lipid raft Liquid-disordered Liquid-ordered Model membrane

More Related Videos

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Published on: September 11, 2019

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Published on: September 16, 2022

Related Experiment Videos

Last Updated: Dec 5, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Published on: September 11, 2019

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Published on: September 16, 2022

Area of Science:

Biophysics
Membrane Biophysics
Physical Chemistry

Background:

Förster resonance energy transfer (FRET) is crucial for studying lipid bilayer heterogeneity.
Current FRET models often assume planar geometry, neglecting vesicle curvature effects.
Unilamellar vesicles (20-200 nm) are common models for membrane studies.

Purpose of the Study:

To develop an analytical model for FRET on spherical lipid bilayers.
To investigate the influence of vesicle size and phase separation on FRET efficiency.
To reconcile theoretical predictions with experimental observations.

Main Methods:

Spherical harmonic expansion of acceptor surface density for analytical FRET solution.
Monte Carlo simulations to validate the theoretical model.
Experimental measurements of FRET efficiency in phase-separated vesicles of varying sizes.

Main Results:

The model accurately predicts FRET efficiency for uniformly distributed molecules on spherical bilayers.
Theoretical predictions indicated FRET efficiency dependence on vesicle size in phase-separated systems due to constrained domain size.
Experimental results did not show a statistically significant change in FRET efficiency with vesicle size in phase-separated vesicles.

Conclusions:

Vesicle size effects on FRET in phase-separated bilayers are theoretically predicted but not experimentally observed.
Factors in extruded lipid vesicle samples likely mask the predicted size-dependent FRET efficiency.
Further investigation is needed to understand the discrepancy between theory and experiment.