A high-stringency blueprint of the human proteome.

Area of Science:

• Proteomics
• Genomics
• Molecular Biology

Background:

• The Human Proteome Organization (HUPO) initiated the Human Proteome Project (HPP) in 2010.
• The HPP aimed to establish global collaboration for proteome data sharing, quality assurance, and accurate genome-encoded proteome annotation.

Purpose of the Study:

• To report the HPP's progress on its tenth anniversary.
• To present a high-stringency human proteome blueprint.
• To demonstrate the utility of proteome knowledge in understanding and treating diseases.

Main Methods:

• Established international collaborations and developed guidelines and metrics.
• Reanalyzed previously deposited community data.
• Increased the coverage of the human proteome through ongoing efforts.

Main Results:

• Achieved a 90.4% complete high-stringency human proteome blueprint.
• Demonstrated the essential role of proteome knowledge in discerning molecular processes.
• Highlighted the proteome's potential in understanding, diagnosing, and treating cancers, cardiovascular diseases, and infectious diseases.

Conclusions:

• The HPP has significantly advanced human proteome coverage over a decade.
• The comprehensive human proteome blueprint is vital for biomedical research.
• Proteomics data is fundamental for progress in human health and disease management.