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Related Experiment Video

Updated: Dec 5, 2025

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
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Ethanol determination in fermented sugarcane substrates by a diffusive micro-distillation device.

Marcos A S Brasil1, Luiz H Gomes2, Marcos Y Kamogawa2

  • 1Center of Nuclear Energy in Agriculture, University of São Paulo. Av. Centenário, 303, P.O. Box 96, CEP: 13400-970 Piracicaba, SP, Brazil.

Journal of Microbiological Methods
|October 17, 2020
PubMed
Summary
This summary is machine-generated.

A new diffusive micro-distillation device (DMDD) accurately measures ethanol in beverages. This method simplifies monitoring fermented substrates, impacting distilled beverage production profitability.

Keywords:
Alcoholic fermentationBioethanol determinationEthanol diffusionUV–vis spectrophotometry

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Area of Science:

  • Analytical Chemistry
  • Chemical Engineering

Background:

  • Ethanol determination is crucial for distilled beverage production profitability.
  • Existing methods can be complex or time-consuming for direct beverage analysis.

Purpose of the Study:

  • To introduce a novel diffusive micro-distillation device (DMDD) for direct ethanol determination in fermented and distilled beverages.
  • To validate the DMDD method against official analytical procedures.

Main Methods:

  • Utilized a simple setup involving a 5 mL test tube with reagent solution inside a 50 mL polyethylene tube containing the sample.
  • Employed a 15-minute water bath heating at 80°C for ethanol diffusion and reaction with potassium dichromate.
  • Quantified the resulting chromium (III) product spectrophotometrically at 589 nm.

Main Results:

  • The proposed DMDD method demonstrated a linear range of 1-12% (v/v) ethanol.
  • Achieved excellent linearity (R² = 0.999) and precision (RSD = 3.8%).
  • Results showed strong agreement with the official distillation-densitometry method.

Conclusions:

  • The DMDD offers a reliable and efficient alternative for direct ethanol quantification in beverages.
  • This method can aid in optimizing distilled beverage production processes.
  • The simplicity and accuracy of DMDD make it suitable for routine monitoring.