Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro testing for carcinogens and mutagens.

R M Santella

    Occupational Medicine (Philadelphia, Pa.)
    |January 1, 1987
    PubMed
    Summary

    In vitro assays provide rapid genotoxic potential information for many chemicals. A battery of complementary tests is recommended to minimize false negatives and assess chemical carcinogenicity risk.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Gene-specific promoter methylation is associated with micronuclei frequency in urothelial cells from individuals exposed to organic solvents and paints.

    Journal of exposure science & environmental epidemiology·2015
    Same author

    Effects of a green tea extract, Polyphenon E, on systemic biomarkers of growth factor signalling in women with hormone receptor-negative breast cancer.

    Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2014
    Same author

    Expression of cytochrome P450 1A1/2 and 3A4 in liver tissues of hepatocellular carcinoma cases and controls from Taiwan and their relationship to hepatitis B virus and aflatoxin B1-and 4-aminobiphenyl-DNA adducts.

    Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals·2013
    Same author

    Mechanism of genotoxicity induced by targeted cytoplasmic irradiation.

    British journal of cancer·2010
    Same author

    Interactions among GSTM1, GSTT1 and GSTP1 polymorphisms, cruciferous vegetable intake and breast cancer risk.

    Carcinogenesis·2007
    Same author

    Hint1 is a haplo-insufficient tumor suppressor in mice.

    Oncogene·2005

    Area of Science:

    • Toxicology
    • Genetics
    • Biochemistry

    Background:

    • Numerous in vitro assays for chemical carcinogenicity and mutagenicity have been developed and validated.
    • These assays are crucial for assessing the potential risks associated with chemical exposure.

    Purpose of the Study:

    • To outline the necessity and application of in vitro assay batteries for evaluating chemical carcinogenicity and mutagenicity.
    • To guide the selection and implementation of tiered testing protocols based on human exposure levels.

    Main Methods:

    • Utilizing a battery or tiered testing protocol of diverse in vitro assays, including bacterial/mammalian mutation, cell transformation, DNA repair, and chromosome assays.
    • Validating assays with known carcinogens and noncarcinogens.
    • Determining the extent of in vitro testing based on the potential human exposure risk of a chemical.

    Main Results:

    • Individual in vitro assays may yield false negatives; therefore, a comprehensive battery is essential for reliable risk assessment.
    • The size and scope of the assay battery should be proportional to the level of potential human exposure.
    • In vitro results inform decisions regarding the need for subsequent animal bioassays.

    Conclusions:

    • In vitro assays offer a rapid method for obtaining significant information on the genotoxic potential of numerous chemicals.
    • While not proving safety, these assays are invaluable for preliminary risk evaluation and prioritizing chemicals for further testing.
    • A combination of complementary in vitro assays is crucial for a robust assessment of chemical carcinogenicity and mutagenicity.

    Related Experiment Videos