Pseudomonas laoshanensis sp. nov., isolated from peanut field soil

A novel Gram-stain-negative, aerobic strain, designated Y22^T, was isolated from peanut field soil in Laoshan Mountain in China. Cells of strain Y22^T were rod-shaped and motile by a single flagellum. The strain was found to be oxidase- and catalase-positive. 16S rRNA gene sequence based on phylogenetic analysis indicated that strain Y22^T belonged to the genus Pseudomonas, and showed the highest 16S rRNA gene sequence similarity of 99.0% to Pseudomonas pelagia JCM 15562^T, followed by Pseudomonas salina JCM 19469^T (98.4%), Pseudomonas sabulinigri JCM 14963^T (97.9%), Pseudomonas bauzanensis CGMCC 1.9095^T (97.6%) and Pseudomonas litoralis KCTC23093^T (97.5%). The phylogenetic analysis based on multilocus sequence analyses with concatenated 16S rRNA, gyrB, rpoD and rpoB genes indicated that strain Y22^T belonged to Pseudomonas pertucinogena lineage. The average nucleotide identity scores between strain Y22^T and closely related species were 74.6-82.8%, and the Genome-to-Genome Distance Calculator scores were 16.4-44.9%. The predominant cellular fatty acids of strain Y22^T were C_18:1ω7c (29.6%), C_17:0 cyclo (17.5%) and summed feature 3 (C_16:1ω7c and/or C_16:1ω6c) (17.4%). The genomic DNA G+C content was 57.9 mol%. On the basis of phenotypic characteristics, phylogenetic analyses and in silico DNA-DNA relatedness, a novel species, Pseudomonas laoshanensis sp. nov. is proposed. The type strain is Y22^T (= JCM 32580^T = KCTC 62385^T = CGMCC 1.16552^T).