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Updated: Dec 5, 2025
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
[Rapid identification of 15 common S. enterica Salmonella serovars by multiplex real-time PCR]
Le Zuo1, Yixiang Jiang1, Min Wan1
1Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Objective:
Multiplex real-time PCR for the identification of 15 Salmonella serovars was developed.
Methods:
Through the Salmonella genome comparison, 12 membrane proteins STM4497 gene can be used to identify 15 Salmonella serovars, and these 12 genes were respectively listed as A-L genes. Then primers were designed according to A-L gene conserved sequences, and then multiplex real-time PCR was established assessed with the evaluation of the limit detection, sensitivity, specificity, and repeatability. The 206 Salmonella strains were identified using multiplex real-time PCR with the comparison of the serum slide agglutination assay.
Results:
The limit detection of multiplex PCR ranged from 1. 1×10~(-3)-1. 2×10~(-3) ng/μL. The target genes were 100% specificity, and the relative standard deviation was lower than 2. 97%. Compared with the serum slide agglutination assay, Kappa ranged 0. 92-1. 00.
Conclusion:
The multiplex real-time PCR can be used to identify 15 Salmonella serovars, which is rapid, accurate and specific.
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