Andrographolide sulfate inhibited NF-κB activation and alleviated pneumonia induced by poly I:C in mice
1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 163 Xianlin Avenue, Nanjing, 210023, China.
Abstract:
Pneumonia is a common illness that continues to be the major killer of remaining to be a significant source of morbidity and mortality in the patient population. Many microorganisms cause pneumonia, and now concern is turning to the importance of the cause the new therapies for viral pneumonia. In the current study, we report the effect of andrographolide sulfonate, a water-soluble form of andrographolide (trade name: Xi-Yan-Ping Injection), on poly I: C-induced pneumonia. Andrographolide sulfonate was administrated through intraperitoneal injection to mice with poly I: C-induced pneumonia. Recruitment of airway inflammatory cells, alteration of lung histological induced by Poly I: C were significantly ameliorated by andrographolide sulfonate. The protein levels of pro-inflammatory cytokines in bronchoalveolar fluid (BALF) and serum were reduced by andrographolide sulfonate treatment. The levels of MUC5AC and MUC5B in lung tissue were also suppressed. These results reveal that andrographolide sulfate remarkably alleviated pneumonia induced by poly I:C in mice. Moreover, andrographolide sulfonate markedly inhibited the activation of nuclear factor-κB (NF-κB). Taken together, we demonstrated that andrographolide sulfonate ameliorated poly I: C-induced pneumonia in mice, suggesting the possible use of andrographolide sulfonate for virus-induced pneumonia in clinical.
Insights
Andrographolide sulfonate effectively treats poly I:C-induced pneumonia in mice by reducing inflammation and improving lung health. This suggests its potential as a therapy for viral pneumonia.
Area of Science:
- Pharmacology
- Immunology
- Respiratory Medicine
Background:
- Pneumonia remains a leading cause of death globally.
- Viral pneumonia presents a growing therapeutic challenge.
- Andrographolide is a compound with potential medicinal properties.
Purpose of the Study:
- To investigate the efficacy of andrographolide sulfonate against poly I:C-induced pneumonia in a mouse model.
- To elucidate the underlying mechanisms of andrographolide sulfonate's action.
Main Methods:
- Polyriboinosinic:polyribocytidilic acid (Poly I:C) was used to induce pneumonia in mice.
- Andrographolide sulfonate was administered via intraperitoneal injection.
- Inflammatory cell recruitment, lung histology, cytokine levels, MUC5AC/MUC5B expression, and NF-κB activation were assessed.
Main Results:
- Andrographolide sulfonate significantly reduced airway inflammatory cell infiltration and lung tissue damage.
- Treatment suppressed pro-inflammatory cytokine levels in bronchoalveolar lavage fluid (BALF) and serum.
- Andrographolide sulfonate inhibited MUC5AC and MUC5B expression and suppressed NF-κB activation.
Conclusions:
- Andrographolide sulfonate demonstrates significant therapeutic effects against poly I:C-induced pneumonia in mice.
- The compound alleviates lung inflammation and mucus production.
- These findings support the potential clinical application of andrographolide sulfonate for treating virus-induced pneumonia.
