Determination of hydrogen peroxide released from cancer cells by a Fe-Organic framework/horseradish...

Multi-walled carbon nanotubes (MWCNTs) were used as conductive carrier on the glassy carbon electrode (GCE), and the hybrid of metal organic framework [NH₂-MIL-53(Fe)] and horseradish peroxidase (HRP) was prepared by simple physical mechanical mixture. The GCE modified by the above material with immobilization, namely NH₂-MIL-53(Fe)/HRP/MWCNTs/GCE, was used to construct an electrochemical biosensor toward H₂O₂. The results indicated that the addition of NH₂-MIL-53(Fe) had a good synergistic effect on the electron transfer of HRP and the detection of H₂O₂. Under the optimized condition, the biosensor exhibited excellent electrochemical performances such as low detection limit, high sensitivity, good stability and so on. The H₂O₂ biosensor showed two linear ranges of 0.1-1 μM and 1-600 μM with a calculated detection limit of 0.028 μM (signal-to-noise ratio, S/N = 3). In addition, the stability of the hybrid of NH₂-MIL-53(Fe) and HRP were discussed by SEM, XRD and UV-vis methods. Furthermore, the reported biosensors were practically used in direct detection of H₂O₂ released from HeLa and HepG2 cells successfully. Thus, this work provides a new strategy to fabricate electrochemical biosensors using MOFs and biomolecules.