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New detection method for uropepsinogen (PGA) using isoelectric focusing and immunoblotting techniques
Zeitschrift Fur Rechtsmedizin. Journal of Legal Medicine
|January 1, 1987
Abstract:
Uropepsinogen (PGA) was isolated and purified from human urine using a column chromatography series. The purified PGA was injected into a rabbit and a PGA-specific antibody was obtained. PGA isozymogen in human urine could be detected reproducibly by immunoblotting using this antibody after isoelectric focusing electrophoresis (IEF) on polyacrylamide gels. This technique may prove to be useful in the genetic study of PGA polymorphism.