Fluvo-metricell, a combined cell volume and cell fluorescence analyzer

Area of Science:

• Biotechnology and Biomedical Engineering
• Cell Biology and Analysis

Background:

• Traditional cell analysis methods often require separate measurements for cell volume and fluorescence.
• Integrating these measurements can improve efficiency and accuracy in cellular studies.

Purpose of the Study:

• To introduce a new flow-through instrument for simultaneous measurement of cell volume and fluorescence.
• To demonstrate the instrument's capability for accurate and coordinated cellular analysis.

Main Methods:

• Utilizes the resistance pulse technique for cell volume measurement.
• Employs axial optical detection of fluorescence pulses from hydrodynamically focused cells.
• Incorporates a novel transducer to eliminate coordination issues between volume and fluorescence signals.

Main Results:

• The instrument accurately measures cell volumes between 4 and 1400 μm³ using a 70-micron orifice.
• Achieves coefficients of variation below 2% for fluorescence measurements.
• Demonstrates successful application through various practical examples and function tests.

Conclusions:

• The developed instrument offers a robust solution for simultaneous, high-precision cell volume and fluorescence analysis.
• This technology enhances cellular analysis by providing coordinated, multi-parametric data.
• The instrument is suitable for diverse applications in cell biology and biotechnology.