Related Experiment Video

Updated: Dec 5, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.0K

LncRNA-SNHG7 interferes with miR-34a to de-sensitize gastric cancer cells to cisplatin

Li-Juan Pei1,1, Peng-Jun Sun1,1, Kui Ma2,1

  • 1Department of General Surgery, The Fourth Medical Center of PLA General Hospital, Beijing, China.

Cancer Biomarkers : Section a of Disease Markers
|October 19, 2020
PubMed

Insights

Gastric cancer (GC) cisplatin resistance is linked to increased SNHG7. Targeting SNHG7 and its miR-34a/LDHA-glycolysis axis can overcome chemoresistance in GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Gastric cancer (GC) exhibits poor prognosis, often due to chemotherapy resistance, particularly to cisplatin.
  • Long non-coding RNAs (lncRNAs) play critical roles in cancer progression, with SNHG7 identified as an oncogenic molecule.

Purpose of the Study:

  • To investigate the role of lncRNA-SNHG7 in cisplatin resistance in gastric cancer.
  • To elucidate the regulatory mechanism involving SNHG7, miR-34a, and LDHA in GC chemoresistance.

Main Methods:

  • Quantitative real-time PCR to measure SNHG7 and miR-34a expression.
  • Cell viability assays to assess cisplatin sensitivity.
  • Bioinformatical analysis and luciferase reporter assays to confirm interactions.
  • Glycolysis rate assays and Western blotting for protein expression.

Main Results:

  • SNHG7 was significantly upregulated in GC tissues and cisplatin-resistant cells, correlating with poor prognosis.
  • Silencing SNHG7 sensitized GC cells to cisplatin, while its overexpression enhanced resistance.
  • SNHG7 negatively regulated miR-34a, which was downregulated in GC.
  • SNHG7 promoted glycolysis by targeting the miR-34a/LDHA axis, and inhibiting SNHG7 reversed cisplatin resistance.

Conclusions:

  • SNHG7 promotes cisplatin resistance in gastric cancer by upregulating glycolysis via the miR-34a/LDHA pathway.
  • The SNHG7/miR-34a/LDHA axis represents a potential therapeutic target for overcoming cisplatin resistance in GC.
Keywords:
Gastric cancerWarburg effectcisplatin resistancelncRNA-SNHG7miR-34a

More Related Videos

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

917
Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells05:27

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

2.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.5K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.5K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.0K
Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists01:28

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy.  SP binds and activates...
411