A new quantitative gel electrophoresis method with image-based detection for the determination of food dyes and...

This work describes an alternative application of gel electrophoresis for the separation and quantification of analytes with low molecular weights using an innovative and low-cost apparatus that enables the acquisition of image-based electropherograms with a webcam. As a proof of concept, the determination of Cu and Ni content in metallic alloys was evaluated by means of the separation and detection of the metallic ions, previously complexed with Eriochrome Black T. Furthermore, the determination of the food colouring agents Sunset Yellow FCF, Tartrazine, Brilliant Blue FCF and Amaranth Red in powder refreshment samples was investigated as alternative to well-stablished methods used for this purpose. For all investigated analytes, the corresponding electrophoretic peaks showed signal to noise ratios ranging from 10 to 180, suitable precision on areas (RSD < 3.5%) and linear relationships (R > 0.99) between RGB detected signals and concentrations of the standard solutions. Application of the method to the determination of Cu and Ni contents in metallic alloys provided results with no significant differences, at 95% confidence level, when compared to the results obtained with a FAAS based method. Apparent recoveries estimated for powder refreshment samples fortified with the food dyes ranged from 93% to 108% for added and found contents, suggesting the absence of matrix effects. The studies prove the feasibility of separation and quantification of coloured analytes by gel electrophoresis and image-based detection that can be useful for different samples.