Peroxidase-like Au@Pt nanozyme as an integrated nanosensor for Ag⁺ detection by LSPR spectroscopy

Here we report the peroxidase-like Au@Pt nanozyme as an integrated nanosensor for selective detection of silver ions (Ag⁺), where the nanozyme plays the roles as both the signal trigger and reporter simultaneously. This method relies on two critical chemical reactions, including (1) the unique inhibitory effect of Ag⁺ on the nanozyme triggered H₂O₂ decomposition at weak acid environment and (2) H₂O₂ induced Ag⁺ reduction onto the nanozyme surface at basic environment, leading to a blueshift in the localized surface plasmonic resonance wavelength (LSPR λ_max) of the nanosensor. With this simple strategy, we demonstrated the sensitive and selective detection of Ag⁺ over a dynamic range from 0.5 to 1000 μM with a limit of detection (LOD) of 500 nM by UV-visible spectroscopy, which is below the permitted level of Ag⁺ in drinking water by U.S. Environmental Protection Agency (EPA). This method also exhibits satisfying recovery efficiency for Ag⁺ detection both in tap water and spring water from the Yuelu Mountain. With this satisfying sensing performance and excellent stability of nanoprobes, this strategy is promising for the detection of Ag⁺ in environment monitoring and food safety analysis.