Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Adverse Event Profile of Apremilast: Pharmacovigilance Study Based on FDA Adverse Event Reporting System (FAERS).

Experimental dermatology·2026

Same author

An Environmentally Tolerant 5A Hydrogel with Photothermal Effect for Frostbite Treatment.

Gels (Basel, Switzerland)·2026

Same author

Regulatory effects of luteolin on apoptosis and autophagy in lung cancer A549 cells.

Journal of toxicology and environmental health. Part A·2026

Same author

Probabilistic Characterization of City Scale Subsurface Thermal Environment Heterogeneity.

Environmental science & technology·2026

Same author

Aggression and emotional distress in adolescents: a cross-sectional chain mediation model of internet addiction and somatization.

Frontiers in psychiatry·2026

Same author

A Large-Scale Nanocrystal Database with Aligned Synthesis and Properties, Enabling Generative Inverse Design.

ACS nano·2026

Search research articles

Related Experiment Video

Updated: Dec 5, 2025

Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

Published on: May 12, 2023

A mitochondria-specific fluorescent probe for rapidly assessing cell viability.

Jianmei Chen¹, Yurui Xu¹, Ya Gao¹

¹National Laboratory of Solid State Microstructures, Chemistry and Biomedicine Innovation Center, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, 210093, P. R. China.

|October 20, 2020

Summary

Rapidly assess cell viability using novel coumarin-quinoline conjugates (CQCs). These mitochondrial dyes offer a fast, sensitive alternative to the MTT assay for real-time monitoring of cell health in biomedical research.

Keywords:

Cell viability Drug screening Fluorescent probe High specificity Mitochondria

More Related Videos

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

Related Experiment Videos

Last Updated: Dec 5, 2025

Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

Published on: May 12, 2023

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

Area of Science:

Biochemistry
Cell Biology
Biomedical Engineering

Background:

Cell viability assays are crucial for research but common methods like the MTT assay are slow and unreliable.
Mitochondrial activity correlates with cell proliferation and apoptosis, offering a target for real-time monitoring.
Existing assays face challenges including time consumption, poor formazan solubility, and interference from cell extracts.

Purpose of the Study:

To develop a novel, rapid, and sensitive method for assessing cell viability.
To utilize coumarin-quinoline conjugates (CQCs) as cell-permeant mitochondrial dyes for monitoring mitochondrial dynamics.
To establish CQCs as a practical tool for evaluating therapeutic effects and cellular health.

Main Methods:

Synthesis of coumarin-quinoline conjugates (CQCs) as novel mitochondrial dyes.
Assessment of CQC mitochondrial targeting and sensitivity to mitochondrial fission.
Comparison of CQC-based viability assessment with the MTT assay for speed and accuracy.

Main Results:

CQCs demonstrated high specificity and preference for mitochondria.
CQCs effectively monitored mitochondrial dynamics, including fissions under pathological conditions.
Cell viability was accurately assessed by CQCs within 10 minutes, significantly faster than the MTT assay.
CQCs showed high sensitivity and accuracy in evaluating therapeutic effects.

Conclusions:

Coumarin-quinoline conjugates (CQCs) offer a rapid and sensitive method for cell viability assessment.
CQCs provide a valuable tool for real-time monitoring of cell fates by tracking mitochondrial dynamics.
This novel assay is practical for biomedical applications, either alone or in combination with other methods.