Bone marrow transplantation in Polish conditions. A modified method of marrow collection and preparation for...

Marrow is collected from posterior iliac crests using specially developed "Wiktor's" needles and from other sites using commercially available sternal needles into heparinized syringes. Then it is directly injected into collecting flasks through specially designed bone marrow filters. This assures single cell nature of marrow cell suspension which then may be either directly infused into the recipient or processed for red cell or T cell depletion. During collection marrow cell suspension is mixed using magnetic stirrers. The described method allows collection of sufficient number of marrow cells for engraftment at the expense of acceptable risk for the donor. Data for 10 consecutive marrow collections are reported.