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Mycoplasma bovis mbfN Encodes a Novel LRR Lipoprotein That Undergoes Proteolytic Processing and Binds Host

James Y Adamu1, Filimon Mitiku2, Carol A Hartley2

  • 1Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia adamu.james@unimelb.edu.au.

Journal of Bacteriology
|October 20, 2020
PubMed

Insights

This study identifies a Mycoplasma bovis lipoprotein, MbfN, as crucial for bacterial adhesion to host cells. Disrupting mbfN significantly reduced M. bovis adherence, highlighting MbfN

Area of Science:

  • Veterinary Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • Mycoplasma bovis causes significant economic losses in ruminants due to severe infections.
  • Lipoproteins are vital membrane components involved in nutrient acquisition, host interaction, and immune response.
  • Many M. bovis genes lack assigned functions due to low sequence similarity with other bacteria.

Purpose of the Study:

  • To investigate the function of the surface-localized leucine-rich repeat (LRR) lipoprotein encoded by mbfN in M. bovis PG45.
  • To determine the role of MbfN in M. bovis adherence and potential virulence.

Main Methods:

  • Sequence analysis of the mbfN gene and its homologs across different M. bovis strains.
  • In silico analysis to predict MbfN function.
  • In vitro binding assays to assess MbfN's interaction with fibronectin and heparin.
  • In vitro adherence assays using M. bovis PG45 with and without mbfN disruption.

Main Results:

  • MbfN homologs were detected in all tested M. bovis strains, with variations in peptide size potentially due to gene region absence.
  • In silico analysis suggested an adhesion-related function for MbfN.
  • MbfN confirmed to bind fibronectin and heparin.
  • Disruption of mbfN significantly reduced M. bovis PG45 adherence to MDBK cells in vitro.

Conclusions:

  • MbfN functions as an adhesin in M. bovis, mediating binding to extracellular matrix components like fibronectin and heparin.
  • The reduced adherence upon mbfN disruption suggests a role for MbfN in M. bovis virulence.
  • Understanding MbfN's function is crucial for developing prevention strategies against M. bovis infections.
Keywords:
LRRMycoplasma bovisadhesinfibronectin bindingheparin bindingimmunogenicityproteolytic processing

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