Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

15.2K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
15.2K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Farewell Cytometry Part A.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2024
Same author

Recommendations for using artificial intelligence in clinical flow cytometry.

Cytometry. Part B, Clinical cytometry·2024
Same author

Counting rare earth metals.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2023
Same author

The first Tissue OMIP is out and alternative modalities to analyze single cells and particles.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2023
Same author

Best impact factor ever and CPHEN, a new acronym for a successful manuscript type.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2022
Same author

A mini review of recent development of flow cytometry in China.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2022
Same journal

CytoScan: Automated Detection of Technical Anomalies for Cytometry Quality Control.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

The 1st Mediterranean Meeting on Flow Cytometry: Forging New Collaborations Across the Mediterranean and Beyond.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Publication Guidelines for Optimized Multiparameter Immunolabeling Panels (OMIPs).

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
See all related articles

Related Experiment Video

Updated: Dec 5, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

10.2K

Diffractive Beam Shaper for Multiwavelength Lasers for Flow Cytometry.

Yong Han1,2,3, Jingjing Zhao4, Zeheng Jiao1,2,3

  • 1State Key Laboratory of Precision Measurement Technology and Instrument, Tsinghua University, Beijing, China.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|October 20, 2020
PubMed
Summary
This summary is machine-generated.

A novel diffractive beam shaper simplifies multicolor laser illumination for flow cytometry, enabling accurate measurements. This new optical element achieves comparable precision to commercial systems for cell analysis and flow velocity detection.

Keywords:
beam shaperdiffractive optics elementsflow cytometrymultiwavelength

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.1K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.2K

Related Experiment Videos

Last Updated: Dec 5, 2025

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

10.2K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.1K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.2K

Area of Science:

  • Optics and Photonics
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Accurate illumination spot formation is critical for flow cytometer measurement precision and stability.
  • Traditional methods for shaping laser beams, especially multiwavelength lasers, are complex and difficult to calibrate.
  • Existing techniques struggle with simultaneous shaping of multiple laser wavelengths for flow cytometry applications.

Purpose of the Study:

  • To develop a simplified diffractive beam shaper for multicolor lasers in flow cytometry.
  • To create a system capable of producing precisely shaped illumination spots for enhanced measurement accuracy.
  • To demonstrate the effectiveness of the novel beam shaper in a customized microflow cytometer.

Main Methods:

  • Designed and fabricated a diffractive optical element (DOE) combined with a focusing lens.
  • Utilized the DOE to shape multiwavelength laser beams into rectangular spots.
  • Integrated the diffractive beam shaper into a customized microflow cytometer for performance evaluation.

Main Results:

  • The diffractive beam shaper successfully produced rectangular spots with dimensions determined by incident wavelengths.
  • Achieved coefficients of variation (CV) of 3.6-6.5% for optical signals, comparable to commercial instruments (3.3-6.3% CV).
  • Enabled accurate measurement of bead sizes (4-15 μm) and real-time flow velocity detection (0.79-9.50 m/s) with <5% CV.

Conclusions:

  • The developed diffractive beam shaper offers a simple and effective solution for multicolor laser illumination in flow cytometry.
  • The system demonstrates high precision and stability, suitable for advanced cell analysis and fluid dynamics measurements.
  • This technology enhances the flexibility and performance of microflow cytometers for various biological applications.