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MicroRNA-183-5p contributes to malignant progression through targeting PDCD4 in human hepatocellular carcinoma

Xiaohui Duan1, Wei Li1, Peng Hu1

  • 1Research Laboratory of Hepatobiliary Tumor, Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410005, China.

Bioscience Reports
|October 20, 2020
PubMed

Insights

MicroRNA-183-5p (miR-183-5p) is highly expressed in hepatocellular carcinoma (HCC) and promotes tumor growth. Targeting miR-183-5p and its target, programmed cell death factor 4 (PDCD4), may offer new therapeutic strategies for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a prevalent global malignancy.
  • MicroRNAs (miRNAs) play critical roles in cancer development and progression.
  • The specific role of microRNA-183-5p (miR-183-5p) in HCC remains largely unexplored.

Purpose of the Study:

  • To investigate the biological function of miR-183-5p in HCC.
  • To elucidate the underlying molecular mechanisms of miR-183-5p in HCC pathogenesis.
  • To evaluate the potential of miR-183-5p as a therapeutic target in HCC.

Main Methods:

  • Quantitative PCR (qPCR) for miRNA expression analysis in clinical HCC samples.
  • In vitro assays (CCK8, flow cytometry, scratch wound, Transwell) to assess HCC cell behavior.
  • In vivo mouse xenograft models for tumor growth evaluation.
  • Bioinformatics, dual-luciferase reporter, and rescue assays for mechanistic studies.

Main Results:

  • miR-183-5p was significantly upregulated in HCC tissues compared to adjacent normal tissues.
  • High miR-183-5p expression correlated with poorer overall survival in HCC patients.
  • Knockdown of miR-183-5p inhibited HCC cell proliferation, migration, invasion, and tumor growth in vivo.
  • Programmed cell death factor 4 (PDCD4) was identified as a direct target of miR-183-5p.
  • PDCD4 downregulation counteracted the inhibitory effects of miR-183-5p knockdown on HCC phenotypes.

Conclusions:

  • miR-183-5p acts as an oncogene in hepatocellular carcinoma.
  • The oncogenic role of miR-183-5p in HCC is mediated through direct targeting of PDCD4.
  • miR-183-5p and its regulatory pathway involving PDCD4 represent potential therapeutic targets for HCC.
Keywords:
PDCD4hepatocellular carcinomamalignant progressionmicroRNA-183-5ppoor prognosis

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