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Metabolomic Associations with Serum Bone Turnover Markers
Moriah P Bellissimo1,2, Joseph L Roberts3,4, Dean P Jones2,5
1Division of Endocrinology, Metabolism and Lipids, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
Nutrients
|October 21, 2020
Summary
This study reveals that bone turnover markers (procollagen type I N-terminal propeptide and C-terminal telopeptides of type I collagen) are linked to specific metabolic pathways, including energy production, nutrient metabolism, and gut microbiome activity in adults.
Area of Science:
- Metabolomics
- Bone Biology
- Nutritional Science
Background:
- Bone is a dynamic tissue undergoing continuous remodeling.
- Bone turnover markers (BTMs), such as procollagen type I N-terminal propeptide (P1NP) for formation and C-terminal telopeptides of type I collagen (CTX) for resorption, are key indicators of bone metabolism.
- Understanding the metabolic underpinnings of bone turnover is crucial for developing targeted interventions.
Purpose of the Study:
- To investigate the associations between plasma metabolites and metabolic pathways with bone formation (P1NP) and resorption (CTX) markers in adults.
- To identify specific metabolic pathways and nutrients linked to bone turnover.
- To explore the relationship between BTMs and microbiome-related metabolites.
Main Methods:
- Cross-sectional clinical study involving 34 healthy adults.
- Quantification of serum BTMs (P1NP and CTX) using ELISA.
- Ultra-high-resolution metabolomics (HRM) with dual-column liquid chromatography and mass spectrometry to analyze plasma metabolites and pathways.
Main Results:
- Metabolites associated with P1NP were enriched in pathways related to the TCA cycle, pyruvate metabolism, B vitamin metabolism, amino acid metabolism (proline, arginine, glutamate), and vitamin C metabolism.
- Metabolites associated with CTX were enriched in lipid and fatty acid beta-oxidation pathways, and fat-soluble micronutrient pathways (vitamins D and E, bile acid biosynthesis).
- Both P1NP and CTX showed significant relationships with microbiome-related metabolites and macronutrient metabolism (lipid, carbohydrate, amino acid).
Conclusions:
- Plasma metabolic profiles are significantly associated with bone turnover markers in healthy adults.
- Specific metabolic pathways, including energy production, nutrient metabolism, and gut microbiome activity, are linked to bone formation and resorption.
- These findings provide a foundation for future research into aging and clinical populations to inform therapeutic strategies for bone health.
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