Immobilization of synthesized phenyl-enriched magnetic nanoparticles in a fabricated Y-Y shaped micro-channel
Omid Rezvani1, Mahin Hashemi Hedeshi1, Habib Bagheri1
1Environmental and Bio-Analytical Laboratories, Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran.
Abstract:
In this survey, a reliable and applicable Y-Y shaped micro-channel in a microfluidic device was designed and manufactured. A number of micro-scaled hedges were precisely fabricated inside the micro-channel to facilitate the immobilization of synthesized core-shell Fe3O4@SiO2 magnetic nanoparticles (MNPs), functionalized by triethoxyphenylsilane (TEPS) by sol-gel technique. Both sample and reagents were introduced into the microfluidic device by a syringe pump to perform the extraction and desorption steps. The functionalized MNPs were characterized by transmission electron microscopy, X-ray diffraction spectroscopy and Fourier transform infrared spectroscopy. By adopting the strategy of extraction-on-chip using this microfluidic device, we were benefited from implementing the entire analyses with the minimum amount of desorbing solvent, MNPs, and aqueous/fruit juice samples. In contrast to dispersive solid phase extraction, dispersion of MNPs during experiment is prevented by fabrication of micro-scaled hedges in the micro-channel. Consequently the stabilized MNPs are reused for the entire runs. The microfluidic device was successfully exploited as an efficient extracting plateau to evaluate the extraction/desorption capability in analysis of some organophosphorus pesticides (OPPs) as model compounds. Our results indicate that the functionalization of Fe3O4@SiO2 with TEPS, improved their extraction capability due to the existence of phenyl and hydroxyl groups for more efficient π-π and hydrogen bonding interactions. Eventually, μL-scale of the organic solvent was injected into a gas chromatography-mass spectrometry system. The limits of detection (3Sb) and quantification (10Sb) for the OPPs were 0.03-0.1 and 0.1-0.35 ng mL-1, respectively. In addition, the interday and intraday precisions were lower than 5.3% (n = 3). The obtained recovery was 95-99% for water samples and 88-96% for fruit juice samples while satisfactory regression coefficients of 0.9949-0.9991, could be achieved.
