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Experimental autoimmune myositis in SJL/J mice
N L Rosenberg1, S P Ringel, B L Kotzin
1Department of Neurology, V.A. Medical Centre, Denver, Colorado 80220.
Clinical and Experimental Immunology
|April 1, 1987
Summary
Researchers developed a new mouse model for experimental autoimmune myositis (EAM). This model shows that producing anti-muscle antibodies does not predict disease susceptibility in mice.
Area of Science:
- Immunology
- Pathology
- Animal Models
Background:
- Experimental autoimmune myositis (EAM) is a condition affecting skeletal muscle.
- Previous EAM models existed in species other than mice.
Purpose of the Study:
- To establish a murine model for EAM.
- To investigate the immunopathogenesis of inflammatory myopathies.
Main Methods:
- SJL/J mice were injected with muscle homogenate and complete Freund's adjuvant (CFA).
- Histological analysis, direct immunofluorescence, and ELISA were used to assess disease and antibody levels.
- Eight other mouse strains were also tested for susceptibility.
Main Results:
- SJL/J mice developed EAM with muscle fiber necrosis and immune cell infiltration.
- IgG deposition was observed in muscle tissues of affected and unaffected strains.
- Elevated anti-muscle antibody levels did not correlate with histological disease development in all strains.
Conclusions:
- The developed murine model provides a new tool for studying EAM.
- Susceptibility to EAM in mice is not solely determined by anti-muscle antibody production.
- This model may offer insights into human inflammatory myopathies.