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Published on: November 18, 2011
JCAD expression and localization in human blood endothelial cells
Manabu Shigeoka1,2, Satomi Arimoto1, Masaya Akashi1
1Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Insights
Junctional Cadherin 5 Associated (JCAD) protein is found at endothelial cell junctions in human salivary glands. Its expression increases in inflamed tissues, suggesting a role in cardiovascular disease pathology.
Area of Science:
- Endocrinology
- Cardiovascular Research
- Histopathology
Background:
- Junctional Cadherin 5 Associated (JCAD) is an endothelial cell-cell junction protein linked to cardiovascular diseases like atherosclerosis and hypertension.
- Limited immunohistochemical studies exist on JCAD expression and localization in human tissues.
Purpose of the Study:
- To confirm JCAD expression and precise localization in human submandibular gland (SMG) tissues using immunohistochemical staining.
- To investigate the correlation between JCAD expression and pathological conditions in SMGs.
Main Methods:
- Immunohistochemical staining was performed on four human SMG specimens.
- Specimens included one with sialoadenitis (inflammation/fibrosis), one largely normal, and two with irradiation-induced fibrosis.
Main Results:
- JCAD was localized at the blood endothelial cell-cell junctions in human SMGs.
- JCAD expression was notably higher in microvessels and arteries within inflamed areas.
Conclusions:
- The study confirms JCAD localization at endothelial cell-cell junctions in human tissues.
- Pathological conditions, such as inflammation, appear to influence JCAD expression levels.
Background:
Junctional Cadherin 5 Associated (JCAD) is an endothelial, cell-cell junction protein, and its expression is associated with cardiovascular diseases including atherosclerosis and hypertension. However, to date, there are few studies confirming JCAD expression and precise localization in human tissues by immunohistochemical staining.
Methods:
JCAD expression and localization was assessed in four human submandibular gland (SMG) specimens by immunohistochemical staining. One specimen of SMG with sialoadenitis was accompanied by severe inflammation and fibrosis, while the other was largely normal. Other two SMGs were accompanied by severe fibrosis because of irradiation.
Results:
Immunohistochemical analysis of human SMGs revealed JCAD localization at the blood endothelial cell-cell junctions. JCAD expression was more evident in microvessels and arteries in areas affected by inflammation.
Conclusions:
The localization of JCAD at endothelial cell-cell junctions was confirmed in human tissues. JCAD expression may be affected by pathological conditions.
