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Updated: Dec 5, 2025

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Published on: December 23, 2016
Multifunctional, histidine-tagged polymers: antibody conjugation and signal amplification
Yichuan Zhang1, Alessia Gambardella, Muhammed Üçüncü
1EaStCHEM School of Chemistry, University of Edinburgh, EH9 3FJ, Edinburgh, UK. mark.bradley@ed.ac.uk annamaria.lilienkampf@ed.ac.uk.
Abstract:
A polymer scaffold, with multiple reactive centres, was synthesised by RAFT polymerisation and conjugated to the antibody herceptin. A hexahistidine RAFT agent enabled the rapid and simple purification of polymer-protein conjugates, while the tetrazine conjugation strategy allows myriad cargos to be attached and amplified.
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