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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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MeDAS: a Metazoan Developmental Alternative Splicing database.

Zhidan Li1, Yiming Zhang1, Stephen J Bush2

  • 1Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu 610041, China.

Nucleic Acids Research
|October 21, 2020
PubMed
Summary
This summary is machine-generated.

MeDAS is a new database that analyzes RNA-seq data to track alternative splicing events during development across many species. This resource helps researchers study how gene expression changes over time.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Alternative splicing significantly expands the transcriptome, producing functional isoforms crucial for development.
  • Temporal regulation of alternative splicing is vital for precise developmental processes.

Purpose of the Study:

  • To introduce MeDAS, a Metazoan Developmental Alternative Splicing database.
  • To provide quantitative data on developmental alternative splicing across species and time.

Main Methods:

  • Re-analysis of 2232 curated polyA+ RNA-seq libraries.
  • Covering embryonic and post-natal development in 18 diverse metazoan species.
  • Development of an interactive browser for data exploration by species, tissue, or genomic feature.

Main Results:

  • MeDAS offers quantitative data on alternative splicing events across developmental time courses.
  • The database includes broad temporal and taxonomic scope, from C. elegans to humans.
  • Users can visualize gene, transcript, and exon-level splicing dynamics over time.

Conclusions:

  • MeDAS facilitates the study of alternative splicing in a developmental context.
  • The database serves as a valuable resource for identifying developmental splicing trends.
  • MeDAS supports research into the functional roles of alternative splicing in metazoan development.