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Live Cell Imaging of Chromosome Segregation During Mitosis
Published on: March 14, 2018
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A quantitative and semiautomated method for determining misaligned and lagging chromosomes during mitosis
Luciano Gama Braga1,2,3, Diogjena Katerina Prifti1,2,3, Chantal Garand2
1Programme en Biologie cellulaire et moléculaire, Faculté de Médicine Université Laval, Québec, QC G1V 0A6, Canada.
Molecular Biology of the Cell
|October 21, 2020
Summary
Researchers developed new ImageJ2/Fiji tools to precisely measure chromosome misalignment during cell division. These methods quantify defects, aiding the study of chromosome segregation errors and aneuploidy in cancer.
Area of Science:
- Cell Biology
- Genetics
- Cancer Research
Background:
- Accurate chromosome alignment at metaphase is crucial for equal sister chromatid segregation.
- Defective alignment leads to chromosome missegregation and aneuploidy, common in cancer.
- Quantitative tools are needed to study chromosome segregation errors.
Purpose of the Study:
- To develop and validate a method for measuring chromosome misalignment.
- To create ImageJ2/Fiji pipelines for quantifying segregation defects.
- To enable sensitive and systematic analysis of mitotic errors.
Main Methods:
- Utilized analytical geometry to develop a measurement method.
- Generated semiautomated ImageJ2/Fiji pipelines.
- Quantified kinetochore misalignment and lagging chromosomes.
Main Results:
- Validated a novel method for measuring chromosome misalignment indicators.
- Developed flexible ImageJ2/Fiji pipelines for quantitative analysis.
- Established tools for assessing chromosome segregation defects.
Conclusions:
- The developed tools allow sensitive and systematic quantitation of chromosome misalignment.
- These methods will advance the understanding of chromosome segregation errors in mitosis.
- Facilitates research into aneuploidy and its role in cancer development.
