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Evaluation of Apoptosis Pathway of Geraniol on Ishikawa Cells

Betül Kuzu1, Gökhan Cüce1, İlknur Çınar Ayan2

  • 1Department of Histology and Embryology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.

Nutrition and Cancer
|October 22, 2020
PubMed

Insights

Geraniol induces apoptosis in endometrial cancer cells by activating the mitochondrial pathway. This natural compound significantly alters key apoptosis-related gene expression, offering potential therapeutic insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Endometrial cancer is the most prevalent malignancy of the female reproductive system.
  • Geraniol, an acyclic monoterpene alcohol from essential oils, has potential anti-cancer properties.
  • Investigating geraniol's mechanism of action in endometrial cancer is crucial for therapeutic development.

Purpose of the Study:

  • To elucidate the apoptosis-inducing effects of geraniol on Ishikawa endometrial cancer cells.
  • To determine the specific apoptosis pathways modulated by geraniol treatment.
  • To analyze the impact of geraniol on key apoptosis-related gene and protein expression.

Main Methods:

  • Cytotoxicity was assessed using the MTT assay to determine the IC50 dose of geraniol.
  • Immunohistochemistry was performed for Bax and Bcl-2 protein expression, and TUNEL assay for apoptosis detection.
  • Quantitative real-time PCR (RT-qPCR) was employed to analyze mRNA expression of apoptosis-related genes.

Main Results:

  • Geraniol treatment significantly decreased Bcl-2 protein staining while increasing Bax protein staining and TUNEL-positive cells.
  • mRNA expression analysis revealed a significant upregulation of Bax, caspase-3, caspase-8, cytochrome C, and Fas genes.
  • A significant downregulation of the Bcl-2 gene was observed in geraniol-treated cells compared to controls.

Conclusions:

  • Geraniol effectively induces apoptosis in Ishikawa endometrial cancer cells.
  • The study suggests geraniol primarily activates apoptosis through the mitochondrial pathway.
  • Geraniol's modulation of apoptosis-related genes highlights its potential as a therapeutic agent for endometrial cancer.

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