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Antimicrobial Effectiveness

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...

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Related Experiment Video

Updated: Dec 4, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

A fast impedance-based antimicrobial susceptibility test.

Daniel C Spencer¹, Teagan F Paton², Kieran T Mulroney³

¹Department of Electronics and Computer Science, and Institute for Life Science, University of Southampton, Hampshire, SO17 1BJ, UK.

Nature Communications

|October 22, 2020

Summary

This summary is machine-generated.

A novel, label-free antimicrobial susceptibility test (AST) provides results in under an hour. This rapid test analyzes bacterial electrical properties to guide antibiotic prescribing decisions effectively.

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Last Updated: Dec 4, 2025

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Published on: February 14, 2022

Stress-induced Antibiotic Susceptibility Testing on a Chip

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Published on: April 18, 2019

Area of Science:

Microbiology
Biomedical Engineering
Diagnostic Technologies

Background:

Urgent need for rapid antimicrobial susceptibility testing (AST) to combat antibiotic resistance.
Current AST methods can be time-consuming, delaying appropriate antibiotic selection.
Developing fast, accurate diagnostics is crucial for effective antimicrobial stewardship.

Purpose of the Study:

To develop and validate a novel, label-free antimicrobial susceptibility test (AST).
To achieve AST results within one hour using actively dividing bacterial cultures.
To enable informed antibiotic prescribing through rapid phenotypic susceptibility data.

Main Methods:

Utilized microfluidic impedance cytometry for single-cell analysis of bacteria.
Incubated bacterial cultures with antibiotics for 30 minutes.
Measured electrical characteristics of approximately 10^5 cells over 2-3 minutes.

Main Results:

The label-free AST delivered results within one hour.
Electrical characteristics correlated with bacterial phenotypic responses to antibiotics.
Results demonstrated consistency with traditional broth microdilution assays across various antibiotics and bacterial species.

Conclusions:

The developed microfluidic impedance cytometry method offers a rapid, label-free AST.
This assay provides timely phenotypic susceptibility information for guided antibiotic therapy.
The test has potential for widespread clinical application in infectious disease management.