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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Commentary: A Systematic Evaluation of Single Cell RNA-Seq Analysis Pipelines

Koji Kadota^1,2,3, Kentaro Shimizu^1,2,3

¹Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Frontiers in Genetics

|October 22, 2020

Summary

No abstract available in PubMed .

Keywords:

asymmetry/asymmetric bulk RNA-seq differential expression analysis gene expression normalization scRNA-seq transcriptome

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