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β-Glucosidase genes differentially expressed during composting.

Xinyue Zhang1, Bo Ma2,3, Jiawen Liu1

  • 1College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin, 150030 China.

Biotechnology for Biofuels
|October 22, 2020
PubMed
Summary

Microbial communities regulate beta-glucosidase (BGL) gene expression differently for glucose-tolerant and non-glucose-tolerant types to adapt cellulose degradation during composting. This adaptation is crucial for global carbon cycling.

Keywords:
Carbon catabolite repressionCompostDifferential expressionFunctional microbial communityβ-Glucosidase

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Cellulose degradation by microbial communities is vital for global carbon cycling.
  • Beta-glucosidase (BGL) is the rate-limiting enzyme in cellulose breakdown.
  • Understanding BGL gene regulation is key to comprehending cellulose degradation.

Purpose of the Study:

  • To investigate the differential regulation of glucose-tolerant and non-glucose-tolerant BGL genes by microbial communities during composting.
  • To test the hypothesis that BGL-producing communities adapt to changing cellulose degradation conditions through differential gene expression.

Main Methods:

  • Metatranscriptomics analysis of functional microbial communities.
  • DNA library screening of GH1 family beta-glucosidases (BGLs).
  • Investigation of gene expression during natural and inoculated composting.

Main Results:

  • Non-glucose-tolerant BGL genes showed higher regulatory sensitivity than glucose-tolerant BGL genes.
  • During composting's cooling phase, BGL gene regulation differed from endoglucanase and exoglucanase expression.
  • Microbial communities upregulated glucose-tolerant BGL under high glucose (carbon catabolite repression) and suppressed non-glucose-tolerant BGL.

Conclusions:

  • Functional microbial communities employ diverse strategies to regulate BGL gene expression.
  • These regulatory mechanisms facilitate adaptation to environmental shifts during composting.
  • The findings support the hypothesis of differential BGL gene regulation for environmental adaptation.