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Fe-N/C single-atom nanozyme-based colorimetric sensor array for discriminating multiple biological antioxidants.

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A novel single-atom nanozyme sensor array simplifies antioxidant detection. This colorimetric array accurately identifies and quantifies multiple antioxidants, including glutathione, in complex samples like human serum.

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biochemistry

Background:

  • Antioxidants are crucial in biological processes and disease.
  • Individual sensors have limited specificity for multiple antioxidants.
  • Sensor arrays offer enhanced specificity for simultaneous detection.

Purpose of the Study:

  • To develop a simplified and reliable sensor array for simultaneous antioxidant identification and quantification.
  • To utilize a single-atom nanozyme for constructing a multi-channel colorimetric sensor.
  • To validate the sensor array's performance in complex biological matrices.

Main Methods:

  • Fabrication of a single-atom nanozyme (Fe-N/C) with oxidase-mimicking activity.
  • Construction of a triple-channel colorimetric sensor array using Fe-N/C to oxidize TMB, ABTS, and OPD.
  • Application of the sensor array for discriminating and quantifying antioxidants like GSH, l-Cys, AA, UA, and MT.

Main Results:

  • The sensor array successfully discriminated five common antioxidants.
  • Accurate quantification of antioxidants was achieved, with glutathione used as a model.
  • The array demonstrated high performance in identifying 15 blind samples in buffer and human serum.
  • Validation included binary and ternary mixtures, showcasing broad applicability.

Conclusions:

  • A single nanozyme-based sensor array provides a simplified and reliable strategy for simultaneous multi-antioxidant probing.
  • This approach overcomes the limitations of individual sensors for complex biological analysis.
  • The developed sensor array shows promise for clinical diagnostics and biochemical research.