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An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization
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Preservation of neck dissection for histological study

P H Rhys Evans1, D Morgan, L Smallman

  • 1Department of Otolaryngology, Queen Elizabeth Hospital, Birmingham.

The Journal of Laryngology and Otology
|August 1, 1987
PubMed

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Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...