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Cloning and expression of treponema pallidum common antigen (Tp-4) in Escherichia coli K12
P Hindersson1, J D Knudsen, N H Axelsen
1Department of Treponematoses, Statens Seruminstitut, Copenhagen, Denmark.
Abstract:
A library of Treponema pallidum DNA was constructed using a cosmid cloning system. Sixteen hundred Escherichia coli recombinant clones were generated covering the T. pallidum genome with a probability of 99%. Three hundred of the clones were screened for expression of T. pallidum antigens by a modified rocket immunoelectrophoresis technique using a polyspecific antiserum to T. pallidum. One clone was identified which produced the 'common antigen' (CA) of T. pallidum (Tp-4). CA shares epitopes with antigens present in more than 50 different bacterial species, but nothing is known about its structure, function and localization. The recombinant E. coli clone will be of value for a structural analysis of the CA gene.
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