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Apigenin Inhibits Proliferation, Migration, and Invasion of Human Tongue Carcinoma Tca8113 Cells Through Regulating

Yun-Ze Xuan1, Li-Zhi Xu2, Cheng-Ri Jin1

  • 1Department of Dentistry, Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, China.

Current Molecular Medicine
|October 23, 2020
PubMed
Abstract

Insights

Apigenin (API) effectively inhibits tongue cancer cell growth, migration, and invasion. Its mechanism involves downregulating the MAPK signaling pathway, offering potential therapeutic insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Human tongue squamous cell carcinoma (Tca8113) exhibits aggressive proliferation, migration, and invasion.
  • Understanding the molecular mechanisms underlying cancer progression is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the effects of apigenin (API) on Tca8113 cell behavior.
  • To elucidate the potential mechanisms, including the involvement of the MAPK signaling pathway.

Main Methods:

  • Cell proliferation was assessed using MTT assay.
  • Migration and invasion were evaluated by cell scratch and Transwell assays.
  • Protein expression and localization (MAPK1, ERK1/2) were analyzed via immunofluorescence and Western blot.

Main Results:

  • Apigenin significantly inhibited Tca8113 cell proliferation (P<0.01).
  • API treatment markedly reduced cell migration and invasion capabilities (P<0.01).
  • Apigenin decreased the expression of phosphorylated MEKK1 and ERK1/2 proteins (P<0.01).

Conclusions:

  • Apigenin demonstrates potent anti-cancer effects against tongue squamous cell carcinoma Tca8113 cells.
  • The observed effects are potentially mediated through the modulation of the MAPK signaling pathway.
Keywords:
Apigenin (API)ERK.MTTTca8113 cellcell proliferationmitogen-activated protein kinase signaling pathways

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