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Coding-Complete Genome Sequences of 23 SARS-CoV-2 Samples from the Philippines.
John Mark Velasco1,2, Piyawan Chinnawirotpisan3, Khajohn Joonlasak3
1Department of Virology, U.S. Army Medical Directorate-Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand VelascoJM@afrims.org.
Microbiology Resource Announcements
|October 23, 2020
Summary
This study presents the complete genome sequences of 23 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) samples from the Philippines. The D614G mutation was prevalent in most of the sequenced SARS-CoV-2 genomes.
Area of Science:
- Virology
- Genomics
- Epidemiology
Background:
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19).
- Genomic surveillance is crucial for understanding viral evolution and spread.
- The D614G mutation in the spike protein is a significant variant of SARS-CoV-2.
Purpose of the Study:
- To determine the complete genome sequences of SARS-CoV-2 samples from the Philippines.
- To analyze the mutational landscape of these viral genomes.
- To identify the prevalence of key mutations like D614G.
Main Methods:
- Whole-genome sequencing of SARS-CoV-2 from nasopharyngeal and oropharyngeal swabs.
- Bioinformatic analysis for genome assembly and mutation identification.
- Comparative analysis with global SARS-CoV-2 genomic data.
Main Results:
- Coding-complete genome sequences for 23 SARS-CoV-2 samples were successfully generated.
- The D614G mutation in the spike protein was detected in 22 out of 23 sequenced genomes.
- This indicates a high prevalence of the D614G variant in the studied Philippine population.
Conclusions:
- The findings provide valuable genomic data for SARS-CoV-2 in the Philippines.
- The high frequency of the D614G mutation suggests its rapid spread and potential impact on viral transmission.
- Continued genomic surveillance is essential for monitoring SARS-CoV-2 evolution in the region.
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