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Published on: April 10, 2018
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Expression profiles of long non-coding RNAs during fetal lung development
Jin-Xin Shen1, Zhi-Dan Bao2, Wen Zhu1
1Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
Experimental and Therapeutic Medicine
|October 23, 2020
Summary
This study identifies 687 differentially expressed long non-coding RNAs (lncRNAs) during fetal lung development. These findings offer insights into the role of lncRNAs in lung development and neonatal pulmonary diseases.
Area of Science:
- Developmental Biology
- Genomics
- Neonatology
Background:
- Advances in neonatology increase survival of premature infants, leading to more common lung development diseases.
- Few studies have investigated the role of long non-coding RNAs (lncRNAs) in fetal lung development.
Purpose of the Study:
- To identify differentially expressed lncRNAs during fetal lung development.
- To explore the potential role of lncRNAs in the pathogenesis of neonatal pulmonary diseases.
Main Methods:
- Collected fetal right lung tissue samples at different gestational ages (weeks 7-16, 16-25, 25-28).
- Utilized Affymetrix Human GeneChip to identify differentially expressed lncRNAs.
- Performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses.
- Validated expression changes of specific lncRNAs using reverse transcription-quantitative PCR.
Main Results:
- Identified 687 differentially expressed lncRNAs across three embryonic periods.
- Detected 34 significantly upregulated and 12 significantly downregulated lncRNAs (fold-change ≥1.5; P<0.05).
- Validated expression changes of lncRNAs n340848, n387037, n336823, and ENST00000445168 via RT-qPCR, confirming GeneChip results.
Conclusions:
- Novel lncRNAs identified may play crucial roles in fetal lung development.
- These findings provide a foundation for further research into lncRNAs in lung development and neonatal lung diseases.
- Understanding lncRNA involvement could clarify the pathogenesis of neonatal pulmonary diseases.
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