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Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

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Leveraging glycomics data in glycoprotein 3D structure validation with Privateer.

Haroldas Bagdonas¹, Daniel Ungar², Jon Agirre¹

¹York Structural Biology Laboratory, Department of Chemistry, University of York, Wentworth Way, York, YO10 5DD, UK.

Beilstein Journal of Organic Chemistry

|October 23, 2020

Summary

Glycoprotein structural biology faces challenges due to glycan complexity. New glycomics data integrated with Privateer software enhances validation of 3D glycoprotein structures.

Keywords:

Privateer X-ray crystallography electron cryomicroscopy glycoinformatics glycomics

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A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Area of Science:

Structural biology
Glycomics
Biochemistry

Background:

Glycoprotein structural analysis is challenging due to glycan heterogeneity, mobility, and complexity.
X-ray crystallography and cryo-electron microscopy (cryo-EM) face limitations in resolving detailed glycan structures.
Advances in mass spectrometry have enabled deeper insights into glycan composition and linkage details, leading to the field of glycomics.

Purpose of the Study:

To extend the Privateer carbohydrate structure validation software.
To integrate glycomics data from large-scale initiatives into the validation process.
To improve the accuracy and reliability of 3D glycoprotein structure validation.

Main Methods:

Leveraging publicly available glycomics databases and their API interfaces.
Extending the Privateer software to incorporate glycomics data.
Utilizing the enhanced software for validation of 3D glycoprotein structures.

Main Results:

Successful integration of glycomics data into the Privateer software.
Demonstrated significant improvement in the validation of 3D glycoprotein structures.
Enhanced ability to assess glycan composition and linkage details in structural models.

Conclusions:

The integration of glycomics data significantly enhances the validation of 3D glycoprotein structures.
Privateer software, augmented with glycomics information, provides a powerful tool for structural biologists.
This approach addresses key challenges in glycoprotein structural analysis, improving data reliability.