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Directed self-assembly software for single cell deposition.

Samuel C Sklare¹, Winona L Richey², Benjamin T Vinson³

¹Department of Physics and Engineering Physics, Tulane University, New Orleans, LA, 70118, United States of America.

International Journal of Bioprinting

|October 23, 2020

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This study introduces novel graphical user interface (GUI) software to enhance laser direct-write (LDW) bioprinting. The software integrates cell transfer, microbead fabrication, and micromachining for improved tissue engineering and cellular microenvironment design.

Area of Science:

Biotechnology
Tissue Engineering
Biofabrication

Background:

Laser direct-write (LDW) bioprinting offers advanced capabilities for designing cellular microenvironments and fabricating tissue constructs.
Current LDW systems require seamless integration of diverse hardware and software components for full potential realization.

Purpose of the Study:

To present the development of a novel graphical user interface (GUI) software designed to enhance LDW bioprinting functionality.
To improve the integration and control of LDW hardware and software components.

Main Methods:

Development of a novel GUI control software with distinct modules for cell transfer, microbead fabrication, and micromachining.
Integration of software modules to manage complex printing programs and experimental parameters.

Keywords:

CAD/CAM bioprinting GUI software laser direct-write machine learning single-cell printing

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Exploration of possibilities for integrated sensor data management within the LDW system.

Main Results:

The developed GUI software facilitates facile control over cell transfer, microbead fabrication, and micromachining processes.
The software addresses key challenges in LDW bioprinting, including construct scale-up, print speed optimization, and experimental condition management.
The presented control software enables streamlined management of sensor data, enhancing experimental reproducibility.

Conclusions:

The novel GUI software significantly improves the capability and functionality of laser direct-write bioprinting.
Seamless integration of software and hardware components, coupled with sensor data management, is crucial for advancing LDW applications in tissue engineering.
This development paves the way for more sophisticated and controlled biofabrication processes.