Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Highly Selective Interfacial Route to Eight-Functional Sucrose Methacrylate for Biocompatible Scaffold Fabrication.

Polymers·2026
Same author

Computer Vision for Predicting the Efficacy of Neoadjuvant Therapy in Breast Cancer.

Cancers·2026
Same author

SPHERpower: MSC spheroid-based bioequivalent lead to the efficient restoration of the scarred vocal folds.

Stem cell research & therapy·2026
Same author

Sex-Stratified Machine Learning for the Prediction of Post-COVID Condition: A Longitudinal Cohort Study.

Journal of clinical medicine·2026
Same author

Comparison of Fascicular Turnover Flap and Autograft in a Rat Facial Nerve Model.

Journal of clinical medicine·2026
Same author

Liver-on-a-Chip: Searching for a Balance Between Biomimetics and Functionality.

Biosensors·2026

Related Experiment Video

Updated: Dec 4, 2025

Laser-induced Forward Transfer of Ag Nanopaste
08:07

Laser-induced Forward Transfer of Ag Nanopaste

Published on: March 31, 2016

11.6K

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process.

Vladimir Yusupov1, Semyon Churbanov1,2, Ekaterina Churbanova1

  • 1Institute of Photon Technologies, Federal Scientific Research Centre "Crystallography and Photonics," Russian Academy of Sciences, Pionerskaya 2, Troitsk, Moscow, 108840, Russia.

International Journal of Bioprinting
|October 23, 2020
PubMed
Summary

Laser-induced forward transfer (LIFT) printing of hydrogels was optimized by controlling laser fluence for precise droplet transfer. Stable temperatures and a practical algorithm ensured consistent bioprinting parameters.

Keywords:
Hydrogel parametersJet and droplets parametersLIFTLaser-induced forward transferOptimal jetting regime

More Related Videos

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
10:14

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

7.6K
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.4K

Related Experiment Videos

Last Updated: Dec 4, 2025

Laser-induced Forward Transfer of Ag Nanopaste
08:07

Laser-induced Forward Transfer of Ag Nanopaste

Published on: March 31, 2016

11.6K
Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
10:14

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

7.6K
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.4K

Area of Science:

  • Bioprinting and advanced materials science.
  • Laser-based fabrication techniques.

Background:

  • Laser-induced forward transfer (LIFT) is a high-resolution, non-contact printing method with significant bioprinting potential.
  • Common bioprinting hydrogels include hyaluronic acid, methylcellulose, and sodium alginate.

Purpose of the Study:

  • To investigate the influence of laser parameters on the LIFT process for three widely used hydrogels.
  • To establish optimal conditions for precise droplet transfer in bioprinting applications.

Main Methods:

  • Utilized a pulsed infrared laser system (1064 nm) with varying laser fluence (1-5 J/cm²) and a 30 μm spot size.
  • Employed high-speed imaging to analyze transfer regimes and droplet characteristics.
  • Controlled environmental factors like temperature (±2°C) and humidity (50%) to mitigate variations.

Main Results:

  • Identified distinct transfer regimes (no transfer, optimal jetting, high speed, turbulent, plume) as laser fluence increased.
  • Demonstrated a near-linear relationship between laser fluence and transferred droplet size/volume in the optimal jetting regime.
  • Quantified hydrogel drying rates (approx. 8 μm/min) and confirmed stable temperatures minimize viscosity changes.

Conclusions:

  • A practical algorithm was developed for rapid configuration of LIFT bioprinting parameters.
  • Optimizing laser fluence is critical for achieving single-droplet transfer and controlling droplet volume.
  • Environmental stability and tunable parameters enable reproducible hydrogel bioprinting.