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Rpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast

A I Garrido-Godino1, I Gupta2, F Gutiérrez-Santiago1

  • 1Departamento de Biología Experimental-Genética, Universidad de Jaén, Jaén, Spain.

RNA Biology
|October 23, 2020
PubMed

Insights

RNA polymerase II subunit Rpb4 plays a key role in gene expression by coordinating mRNA synthesis, imprinting, and stability. Rpb4 interacts with RNA-binding protein Puf3 to regulate mRNA stability and chromatin association.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Post-transcriptional Modification

Background:

  • Gene expression is tightly regulated by the interplay between mRNA synthesis and degradation.
  • RNA polymerase II (RNA pol II) subunit Rpb4, and its dimer Rpb4/7, are central to this regulation.
  • Understanding the molecular mechanisms of Rpb4 in post-transcriptional regulation is crucial.

Purpose of the Study:

  • To unravel the molecular mechanisms of Rpb4 in regulating mRNA imprinting and stability.
  • To investigate the genome-wide association of Rpb4 with mRNAs.
  • To explore the interaction between Rpb4 and the RNA-binding protein Puf3.

Main Methods:

  • RNA immunoprecipitation sequencing (RIP-Seq) to analyze Rpb4-mRNA association genome-wide.
  • Analysis of physical, genetic, and functional interactions between Rpb4 and Puf3.
  • Assessment of Rpb4 and Puf3 dependency for mRNA association and chromatin binding.

Main Results:

  • Rpb4 associates with over 1400 transcripts, a subset of which are also targeted by Puf3.
  • Rpb4 and Puf3 physically, genetically, and functionally interact, influencing common mRNA stability and imprinting.
  • The association of both Rpb4 and Puf3 with mRNAs is interdependent, and Puf3 chromatin association is Rpb4-dependent.

Conclusions:

  • Rpb4 is a key component of RNA pol II that coordinates mRNA synthesis, imprinting, and stability.
  • Rpb4 cooperates with RNA-binding proteins like Puf3 in post-transcriptional gene regulation.
  • This study provides novel insights into the multifaceted role of Rpb4 in gene expression control.
Keywords:
Puf3RNA polymerase IIRbpSaccharomyces cerevisiaemRNA decaymRNA imprinting

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