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Updated: Sep 11, 2026

Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
Published on: August 3, 2013
[Results of the storage of freeze dried microbial cultures for 25 years]
Abstract:
Saprophytic microorganisms belonging to different physiological groups (Azotobacter, acetic, ammonifying, lactic and nodule bacteria, a phototrophous purple bacterium of the Chromatium genus, bacteria of the Micrococcus and Pseudomonas genera, and a yeast of the Candida genus) were stored at 3-6 degrees C for 25 years in the freeze-dried state. All of the strains were found to be viable after the storage. The number of viable cells decreased for some bacteria, but to a far less degree than when the cultures were kept immediately after the freeze-drying for a year at 30 degrees C.
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