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Updated: Dec 4, 2025
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
Published on: April 6, 2021
Serum pooling for rapid expansion of anti-SARS-CoV-2 antibody testing capacity
J W L Allen1, H Verkerke1, J Owens2
1Department of Laboratory Medicine and Pathology, Center for Transfusion Medicine and Cellular Therapies, Emory University School of Medicine, 201, Dowman Dr, 30322 Atlanta, GA, United States; Department of Pathology, Joint Program in Transfusion Medicine, Harvard Medical School, Brigham and Women's Hospital, 630D New Research Building, 02115 Boston, MA, United States.
Objectives:
Examine possible pooling strategies designed to expand SARS-CoV-2 serological testing capacity.
Methods:
Negative pools were assessed to determine optimal optical density (OD) cutoffs, followed by spiking weak or strong positive samples to assess initial assay performance. Samples were then randomly subjected to pool and individual testing approaches.
Results:
Single positive specimens consistently converted pools of 5, 10, or 20 into positive outcomes. However, weaker IgG-positive samples failed to similarly convert pools of 50 to a positive result. In contrast, a stronger individual positive sample converted all pools tested into positive outcomes. Finally, examination of 150 samples configured into pools of 5, 10, 20 or 50 accurately predicted the presence of positive or negative specimens within each pool.
Conclusions:
These results suggest that pooling strategies may allow expansion of serological testing capacity. While limitations exist, such strategies may aid in large-scale epidemiological screening or identification of optimal convalescent plasma donors.
