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Updated: Dec 4, 2025

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
Published on: April 12, 2014
An automated vertical drift correction algorithm for AFM images based on morphology prediction
Yinan Wu1, Yongchun Fang1, Zhi Fan1
1Institute of Robotics and Automatic Information System, College of Artificial Intelligence, Nankai University, Tianjin, China; Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin, China.
Abstract:
The atomic force microscope (AFM) has become a powerful tool in many fields. However, environmental noise and other disturbances are very likely to cause the AFM probe to vibrate, which lead to vertical drift in AFM imaging and limit its further application. Therefore, to correct image distortion caused by vertical drift, a morphology prediction based image correction algorithm is proposed in this paper. Specifically, a Gaussian-Hann filter is first designed for distorted AFM images, based on which, an adaptive image binarization algorithm is developed to achieve accurate object detection and background extraction. Furthermore, an advanced morphology prediction algorithm, consisting of morphological approximation prediction and morphological detail prediction, is proposed to correct image distortion by using the extracted substrate of a sample image. Approximate morphology is generated by an improved weighted fusion autoregressive model, and morphological detail is obtained by energy analysis based on discrete wavelet transform. Experimental and application results are presented to illustrate that the proposed algorithm is able to effectively eliminate vertical drift of AFM images.
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