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Updated: Dec 4, 2025

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
Published on: January 22, 2015
Enhancing bio-cathodic nitrate removal through anode-cathode polarity inversion together with regulating the anode
Zhufan Lin1, Shaoan Cheng1, Zhen Yu1
1State Key Laboratory of Clean Energy, Department of Energy Engineering, Zhejiang University, Hangzhou 310027, PR China.
A novel polarity inversion method rapidly cultivates high-performance nitrate-reducing bio-cathodes. This approach enhances electrogenic bacteria and nitrate-reducing bacteria biomass, significantly boosting nitrate removal efficiency.
Area of Science:
- Environmental Microbiology
- Electrochemistry
- Biotechnology
Background:
- Bio-cathodic nitrate removal offers a cost-effective solution for nitrate contamination.
- Current methods suffer from long start-up times and suboptimal performance, hindering practical application.
Purpose of the Study:
- To develop a rapid cultivation strategy for high-performance nitrate-reducing bio-cathodes.
- To investigate the impact of anode-cathode polarity inversion on bio-electrochemical activity.
- To explore the correlation between bio-anodic oxidation and bio-cathodic reduction performance.
Main Methods:
- Anode-cathode polarity inversion technique applied under varying external resistances.
- Analysis of bio-electrochemical nitrate removal rates.
- 16S rRNA gene sequencing and bacterial biomass quantification.
Main Results:
- Achieved a high bio-electrochemical nitrate removal rate of 2.74 ± 0.03 gNO3--N m-2 d-1.
- Demonstrated a 4.0-fold increase in removal rate compared to low-activity bioelectrodes.
- Established a linear correlation between reversed bio-anodic oxidation and bio-cathodic nitrate removal performance.
Conclusions:
- The polarity inversion method effectively cultivates high-performance nitrate-reducing bio-cathodes.
- Increased bacterial biomass of electrogenic and nitrate-reducing bacteria drives enhanced removal.
- Findings suggest electrogenic biofilms play a dual role in nitrate reduction and system promotion, enabling functional bio-cathode development.
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