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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Positioning Bacillus subtilis as terpenoid cell factory.

H Pramastya^1,2, Y Song¹, E Y Elfahmi²

¹University of Groningen, Groningen, The Netherlands.

Journal of Applied Microbiology

|October 24, 2020

Summary

Bacillus subtilis is a promising microbial factory for producing valuable terpenoids using the methylerythritol phosphate (MEP) pathway. This review details MEP pathway enzymes, terpene synthases, and engineering strategies for enhanced terpenoid production.

Keywords:

Bacillus subtilis MEP pathway cell factory metabolic engineering terpenoids

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Area of Science:

Biotechnology
Metabolic Engineering
Synthetic Biology

Background:

Microbial production of bioactive compounds is crucial for meeting increasing demands.
Bacillus subtilis is a safe and efficient host for producing complex natural products like terpenoids.
The endogenous methylerythritol phosphate (MEP) pathway in B. subtilis provides high capacity for isoprene precursor biosynthesis.

Purpose of the Study:

To review current knowledge of the MEP pathway and its enzymes in B. subtilis.
To discuss terpene synthases and engineering strategies for improving terpenoid production.
To highlight genetic tools and future directions for developing B. subtilis as a terpenoid cell factory.

Main Methods:

Review of literature on MEP pathway enzymes and terpene synthases in B. subtilis.
Analysis of metabolic engineering strategies for enhancing terpenoid biosynthesis.
Discussion of genetic tools and future prospects for microbial cell factory development.

Main Results:

Detailed description of each enzyme in the MEP pathway and C5 isoprene precursor synthesis.
Systematic discussion of representative terpene synthases and engineering approaches in B. subtilis.
Overview of available genetic tools and promising strategies for terpenoid production improvement.

Conclusions:

Bacillus subtilis is a viable and highly capable host for microbial terpenoid production.
Further metabolic engineering and utilization of genetic tools can significantly enhance B. subtilis's potential as a cell factory.
This review provides insights to inspire future research in developing robust B. subtilis-based terpenoid production systems.