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Stable Plasma Sample Storage in Acetonitrile for Angiotensin and Aldosterone Analysis
Xuefei Wei1, Yanyang Wang1, Wenbo Zhu2
1Department of Nuclear Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu, PR China.
Laboratory Medicine
|October 24, 2020
Summary
A new method using 60% acetonitrile stabilizes plasma samples for angiotensin I, II, and aldosterone, eliminating the need for cold storage and enabling remote sample collection.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Analytical Chemistry
Background:
- Plasma levels of Angiotensin I (AI), Angiotensin II (AII), and aldosterone are unstable at room temperature.
- This instability complicates sample collection for remote regions in collaborative studies.
- A novel, non-cold storage method is needed for reliable analysis.
Purpose of the Study:
- To develop and validate a stable storage method for plasma AI, AII, and aldosterone.
- To assess the stability of these analytes at room temperature using acetonitrile.
- To facilitate remote sample collection and analysis.
Main Methods:
- Plasma samples were treated with 60% acetonitrile.
- Supernatants were stored at 4°C and room temperature for up to 30 days.
- AI, AII, and aldosterone levels were quantified using chemiluminescence immunoassays.
Main Results:
- AI, AII, and aldosterone were well-retained in plasma with 60% acetonitrile.
- The method demonstrated good intra- and inter-day precision (CVs <10%).
- Analyte stability was maintained for 30 days at room temperature.
Conclusions:
- Adding 60% acetonitrile to plasma provides a stable storage solution.
- This method eliminates the requirement for cold chain management.
- It enables reliable clinical analysis of AI, AII, and aldosterone from remote samples.
