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Human Histone Interaction Networks: An Old Concept, New Trends
Yunhui Peng1, Yaroslav Markov2, Alexander Goncearenco3
1National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA.
Journal of Molecular Biology
|October 25, 2020
Summary
This study maps human histone interactions, revealing a complex network with scale-free properties. It identifies conserved and variant-specific binding sites, crucial for understanding chromatin regulation.
Area of Science:
- Molecular Biology
- Systems Biology
- Genomics
Background:
- Histones are fundamental proteins organizing DNA into chromatin.
- Understanding histone interactions is key to deciphering gene regulation and cellular processes.
- Existing data on histone interactions are fragmented across various study types.
Purpose of the Study:
- To create a comprehensive map of the human histone interactome.
- To analyze the topological and binding interface properties of this network.
- To understand how chromatin factors interact with histones.
Main Methods:
- Integration of data from structural, chemical cross-linking, and high-throughput studies.
- Construction of a global human histone interaction network.
- Analysis of network topology and histone binding interfaces.
Main Results:
- A combined network of 5308 proteins and 10,330 interactions was generated.
- The human histone interactome exhibits scale-free behavior and high modularity.
- 80-90% of H3 and H4 residues participate in interactions, with conserved and variant-specific binding modes identified.
Conclusions:
- Histone interactions form a complex, modular network critical for chromatin function.
- Distinct binding modes are utilized by chromatin factors to recognize histones.
- This integrated network provides a valuable resource for future research in epigenetics and disease.
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