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Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p

Juanjuan Shi1,2, Xijian Xu3, Dan Zhang4

  • 1Department of Gynaecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, 107 West Wenhua Road, Jinan, 277599, Shandong, China.

Journal of Ovarian Research
|October 25, 2020
PubMed
Abstract

Insights

Long non-coding RNA PTPRG antisense RNA 1 (PTPRG-AS1) promotes epithelial ovarian cancer (EOC) malignancy by acting as an oncogene. Silencing PTPRG-AS1 inhibits EOC progression and tumor growth via the miR-545-3p/HDAC4 pathway.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNA PTPRG antisense RNA 1 (PTPRG-AS1) is implicated in various cancers.
  • Its specific role and mechanism in epithelial ovarian cancer (EOC) require further elucidation.

Purpose of the Study:

  • To investigate the function of PTPRG-AS1 in EOC development.
  • To identify the molecular mechanisms underlying PTPRG-AS1's role in EOC.

Main Methods:

  • PTPRG-AS1 expression was quantified in EOC tissues and cell lines using RT-qPCR.
  • Functional assays assessed the impact of PTPRG-AS1 silencing on EOC cell behavior in vitro and tumor growth in vivo.
  • Bioinformatics, luciferase reporter, and RNA immunoprecipitation assays identified the PTPRG-AS1/miRNA interaction.

Main Results:

  • PTPRG-AS1 was upregulated in EOC and associated with poorer patient survival.
  • PTPRG-AS1 silencing suppressed EOC cell proliferation, migration, invasion, and tumor growth, while promoting apoptosis.
  • PTPRG-AS1 acts as a competing endogenous RNA (ceRNA) by sponging miR-545-3p, leading to increased HDAC4 expression.

Conclusions:

  • PTPRG-AS1 acts as an oncogenic lncRNA in EOC, promoting malignancy through the miR-545-3p/HDAC4 ceRNA network.
  • Targeting the PTPRG-AS1/miR-545-3p/HDAC4 axis presents a potential therapeutic strategy for EOC.
Keywords:
Anticancer treatmentsNon-coding RNAPTPRG antisense RNA 1ceRNA

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