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Growth of Cartilage and Bone Tissue

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...

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Related Experiment Video

Updated: Dec 4, 2025

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

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DMOG Negatively Impacts Tissue Engineered Cartilage Development.

Jessica M Falcon¹, Dylan Chirman¹, Alyssa Veneziale¹

¹Tissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.

|October 26, 2020

Summary

This summary is machine-generated.

Short-term use of dimethyloxalylglycine (DMOG) did not improve matrix production in engineered cartilage. While DMOG stabilized HIF-1α in some areas, it reduced overall matrix deposition and mechanical properties in 3D cultures.

Keywords:

cartilage collagen mesenchymal stem cells tissue engineering

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Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

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Published on: January 7, 2019

Area of Science:

Biomedical Engineering
Regenerative Medicine
Stem Cell Biology

Background:

Articular cartilage functions in a hypoxic environment.
Hypoxia-simulating agents are explored for cartilage tissue engineering.
Dimethyloxalylglycine (DMOG) stabilizes HIF-1α.

Purpose of the Study:

To investigate if DMOG improves matrix production in 3D engineered cartilage.
To assess DMOG's effect on porcine synovial-derived mesenchymal stem cell (SYN-MSC) and chondrocyte co-cultures.

Main Methods:

3D pellet cultures and scaffold-based engineered cartilage were used.
DMOG treatment was applied from day 14 to day 21.
Constructs were analyzed up to 6 weeks.

Main Results:

DMOG stabilized HIF-1α in the exterior of engineered cartilage.
Increased regional type II collagen deposition was observed.
Overall extracellular matrix deposition and mechanical competency were negatively affected.

Conclusions:

Short-term DMOG treatment did not uniformly enhance chondrogenesis in 3D cultures.
Naturally hypoxic centers in 3D constructs may limit DMOG's benefit.
DMOG's efficacy in cartilage tissue engineering requires further investigation.