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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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Studying the Cytoskeleton01:17

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
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Biological physics by high-speed atomic force microscopy.

Ignacio Casuso1, Lorena Redondo-Morata2, Felix Rico1

  • 1Aix-Marseile University, Inserm, CNRS, LAI, 163 Av. de Luminy, 13009 Marseille, France.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|October 26, 2020
PubMed
Summary
This summary is machine-generated.

High-speed atomic force microscopy (HS-AFM) offers nanometre imaging and piconewton force measurements for biological physics. This technique reveals fast biophysical processes, advancing our understanding of biological systems.

Keywords:
biophysicscellshigh-speed force spectroscopymembranesproteinssingle molecules

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Area of Science:

  • Biological physics
  • Nanotechnology
  • Biophysics

Background:

  • Nanotechnology provides a novel scale for observing biological systems.
  • High-speed atomic force microscopy (HS-AFM) offers nanometre resolution and subsecond dynamics.
  • HS-AFM enables piconewton force measurements within microseconds.

Purpose of the Study:

  • To review the contributions of HS-AFM to biological physics.
  • To highlight HS-AFM's role in imaging and force spectroscopy.
  • To discuss how HS-AFM advances the understanding of fast biophysical processes.

Main Methods:

  • High-speed atomic force microscopy (HS-AFM) in imaging mode.
  • High-speed atomic force microscopy (HS-AFM) in force spectroscopy mode.
  • Analysis of biological systems at the nanoscale.

Main Results:

  • HS-AFM provides nanometre structural and dynamic information of biological systems.
  • HS-AFM measurements reveal fast biophysical processes previously inaccessible.
  • Observations of membrane remodelling, molecular motors, and protein unfolding have been achieved.

Conclusions:

  • HS-AFM significantly contributes to biological physics by enabling observation of emergent physical phenomena.
  • HS-AFM has stimulated novel theories and concepts in biophysics.
  • Future applications of HS-AFM will further bridge the gap between biology and physics.